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| Registros recuperados: 36 | |
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| Kuboki, Noritaka; Tiwananthagorn, Weerawan; Takagi, Hideaki; Nakayama, Tomoko; Xuan, Xuenan; Inoue, Noboru; Igarashi, Ikuo; Tsujimura, Kunio; Ikehara, Yuzuru; Kojima, Naoya; Yokoyama, Naoaki. |
| The oligomannose-coated liposome (OML) vaccine is known to induce cellular immunity specific for the encapsulated antigen in immunized mice. In the present study, we preliminarily evaluated the effect of the OML vaccine encapsulating the soluble protozoan lysate of Toxoplasma gondii, Trypanosoma brucei gambiense, or Babesia rodhaini on the corresponding protozoan infections in mice. After the challenge of T. gondii, the OML vaccine group avoided the high mortality resulting from acute infection that was dominantly observed in other control groups. During the infectious course, the development of the T. gondii-specific antibody, which is an indicator of humoral immunity, was constantly controlled at a lower level in the surviving mice of the OML vaccine... |
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Palavras-chave: Oligomannose-coated liposome (OML); OML; Soluble protozoan antigen; Antibody responses. |
| Ano: 2007 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1050 |
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| Namangala, Boniface; Inoue, Noboru; Sugimoto, Chihiro; 井上, 昇. |
| Transforming growth factor beta-1 (TGF-β1) is a pleiotropic cytokine with both pro- and antiinflammatory properties, depending on its environment and concentration. The present study evaluated the effects of orally-delivered TGF-β1 on mice parenterally-infected with various protozoan parasites. We report that while orally-administered TGF-β1 seems to confer partial protection against murine chronic babesiosis and acute trypanosomosis, no beneficial clinical effects were observed against acute babesiosis, malaria or toxoplasmosis. Taken together, these preliminary data suggest that the systemic effects conferred by exogenous TGF-β1 could be parasite speciesspecific. The variations in different parasitic infections could be due to (i) intrinsic differences... |
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Palavras-chave: Low-dose; Mice; Orally-delivered TGF-β1; Protection; Protozoan parasites. |
| Ano: 2009 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2716 |
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| Seng, Seyha; Maki, Yoshiyuki; Yokoyama, Minesuke; Inoue, Noboru; Igarashi, Ikuo; Nagasawa, Hideyuki; Horiuchi, Motohiro; Omata, Yoshitaka; Saito, Atsushi; Suzuki, Naoyoshi; Toyoda, Yutaka; 井上, 昇; 五十嵐, 郁男; 長澤, 秀行. |
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Palavras-chave: Toxoplasma gondii; SAG-1 (p30); Transgenic mice. |
| Ano: 1996 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/254 |
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| Fereig, Ragab M.; Mohamed, Samy G. A.; Mahmoud, Hassan Y. A. H.; AbouLaila, Mahmoud Rezk; Guswanto, Azirwan; Thu-Thuy Nguyen; Mohamed, Adel Elsayed Ahmed; Inoue, Noboru; Igarashi, Ikuo; Nishikawa, Yoshifumi. |
| Babesia bovis, B. bigemina, Trypanosoma evansi, and Anaplasma marginate infections cause serious diseases in cattle, and are primarily transmitted by arthropod vectors (ticks for B. bovis, B. bigemina, and A. marginate and various types of flies for T. evansi). In the last few years, there have been many reports of a high prevalence of certain protozoan infections in northern Egypt, but no accurate or adequate data are available for the southern regions. Therefore, in this study, we screened for evidence of such diseases in economically important cattle species using serum samples. The seroprevalence of protozoan infections in cattle was determined with enzyme-linked immunosorbent assays using species-specific diagnostic antigens. In a total of 301 cattle... |
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Palavras-chave: Babesia bovis; Babesia bigemina; Trypanosoma evansi; Anaplasma marginale; Cattle; Egypt. |
| Ano: 2017 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4579 |
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| Thekisoe, Oriel M. M.; Kuboki, Noritaka; Nambota, Andrew; Fujisaki, Kozo; Sugimoto, Chihiro; Igarashi, Ikuo; Yasuda, Jun; Inoue, Noboru. |
| In this study, we developed loop-mediated isothermal amplification (LAMP) for the specific detection of both animal and human trypanosomosis using primer sets that are designed from 5.8S rRNA-internal transcribed spacer 2 (ITS2) gene for Trypanosoma brucei gambiense, 18S rRNA for both T. congolense and T. cruzi, and VSG RoTat 1.2 for T. evansi. These LAMP primer sets are highly sensitive and are capable of detecting down to 1 fg trypanosomal DNA, which is equivalent to 0.01 trypanosomes. LAMP is a rapid and simple technique since it can be carried out in 1 h and requires only a simple heating device for incubation. Therefore, LAMP has great potential of being used for diagnosis of trypanosomosis in the laboratory and the field, especially in countries that... |
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Palavras-chave: LAMP; Trypanosomosis; Trypanosoma brucei brucei; T. b. rhodesiense; T. b. gambiense; T. congolense; T. cruzi; T. evansi. |
| Ano: 2007 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1045 |
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| Suganuma, Keisuke; Yamasaki, Shino; Molefe, Nthatisi Innocentia; Musinguzi, Peter Simon; Davaasuren, Batdorj; Mossaad, Ehab; Narantsatsral, Sandagdorj; Battur, Banzragch; Battsetseg, Badgar; Inoue, Noboru. |
| Dourine is caused by Trypanosoma equiperdum via coitus with an infected horse. Although dourine is distributed in Equidae worldwide and is listed as an internationally important animal disease by the World Organization for Animal Health (OIE), no effective treatment strategies have been established. In addition, there are no reports on drug discovery, because no drug screening system exists for this parasite. A new T.?equiperdum strain was recently isolated from the genital organ of a stallion that showed typical symptoms of dourine. In the present study, we adapted T.?equiperdum IVM-t1 from soft agarose media to HMI-9 liquid media to develop a drug screening assay for T.?equiperdum. An intracellular ATP-based luciferase assay using CellTiter-Glo reagent... |
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Palavras-chave: Colorimetric assay; Drug screening system; Liquid culture; Luciferase assay; Trypanosoma equiperdum. |
| Ano: 2017 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4538 |
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| Buates, S.; Xuan, Xuenan; Igarashi, Makoto; Sugimoto, Chihiro; Inoue, Noboru; 玄, 学南; 五十嵐, 慎; 井上, 昇. |
| Toxoplasma gondii microneme protein 2 (TgMIC2), an apically stored adhesin, was shown to be a key participant involved in the initial attachment and invasion to a host cell. In this study, we had established the clonal line of T. gondii tachyzoites which over-expressed TgMIC2 using the tetracycline repressor (TetR)-based inducible gene expression system. The TgMIC2 over-expression significantly reduced tachyzoite propagation in vitro (p < 0.002) and significantly reduced the parasite virulence in mice (p = 0.002). The over-expression of exogenous TgMIC2 caused the increase of mRNA expression level of endogenous TgMIC2. Wild type parasites mainly expressed TgMIC2115 protein, the microneme store and the surface form of TgMIC2. Over-expression of TgMIC2... |
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Palavras-chave: Toxoplasma gondii; TgMIC2; Over-expression. |
| Ano: 2008 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2236 |
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| Tuvshintulga, Bumduuren; Sivakumar, Thillaiampalam; Battsetseg, Badgar; Narantsatsaral, Sandag-ochir; Enkhtaivan, Batsaikhan; Battur, Banzragch; Hayashida, Kyoko; Okubo, Kazuhiro; Ishizaki, Takahiro; Inoue, Noboru; Igarashi, Ikuo; Yokoyama, Naoaki. |
| Babesia microti is a tick-transmitted zoonotic hemoprotozoan parasite. In the present study, we investigated B. microti infection in questing ticks in Mongolia. A total of 219 questing ticks were collected from three different Mongolian provinces (Bayan-Olgii, Khovsgol, and Selenge). Of these, 63 from Selenge were identified as Ixodes persulcatus, while the remaining 156 (from all three provinces) were identified as Dermacentor nuttalli. When the tick DNA samples were screened using a B. microti-specific nested PCR, 19 (30.2%) of the 63 I. persulcatus ticks were found to be B. microti-positive. The parasite was not detected in D. nuttalli. Subsequently, the 18S rRNA, cox1, and tufA sequences of B. microti were amplified, sequenced, and subjected to... |
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Palavras-chave: 18S rRNA; B. microti; Cox1; Mongolia; Ticks; TufA. |
| Ano: 2015 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4554 |
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| Kamada, Takenori; Igarashi, Ikuo; Inoue, Noboru; Nagasawa, Hideyuki; Suzuki, Hiroshi; Kodama, Tatsuhiko; Suzuki, Naoyoshi; Toyoda, Yutaka; 五十嵐, 郁男; 井上, 昇; 長澤, 秀行; 鈴木, 宏志. |
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Palavras-chave: Babesiosis; Protective immune response; Knock-out mouse; SRKO. |
| Ano: 1997 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/265 |
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| Kato, Mihoko; Maki, Yoshiyuki; Inoue, Noboru; Omata, Yoshitaka; Claveria, Florencia G.; Igarashi, Ikuo; Saito, Atsushi; Suzuki, Naoyoshi; 井上, 昇; 五十嵐, 郁男. |
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Palavras-chave: Cytotoxic cell activity; NK cell; LAK cell; Toxoplasma lysate antigen. |
| Ano: 1994 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/216 |
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| Kuboki, Noritaka; Kibe, Michael K; Thekisoe, Oriel M. M.; Sugimoto, Chihiro; Inoue, Noboru. |
| With the hypothesis that African trypanosomes could have in vivo specific genes for adaptation to host’s environment, the present study was conducted by using suppressive subtractive hybridization (SSH) technique to seek the highly expressed genes especially in host. A total of 328 clones from the in vivo SSH library and that of 160 clones from the in vitro SSH library were analyzed in order to determine their expression levels, but none of the above-mentioned genes showed differential expression. This indicates that no trypanosome genes could be differentially expressed either the in vivo or in vitro propagated trypanosomes. Alternatively, there might be limitation for detecting specifically expressed genes in African trypanosomes using this method,... |
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Palavras-chave: Differential expression; Infection; Trypanosoma brucei. |
| Ano: 2007 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1052 |
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| Mossaad, Ehab; Salim, Bashir; Suganuma, Keisuke; Musinguzi, Peter; Hassan, Mohammed A.; Elamin, E. A.; Mohammed, G. E.; Bakhiet, Amel O.; Xuan, Xuenan; Satti, Rawan A.; Inoue, Noboru. |
| Background: This study was conducted in response to recurring reports from eastern Sudan of camel trypanosomosis that can no longer be treated by currently available trypanocidal drugs. One hundred and eighty-nine blood samples were obtained from camels in different herds and local markets in the western part of Sudan, and a cross-sectional study was carried out between December 2015 and February 2016 to identify the causative agents and possible circulating genotypes. Results: The prevalence of trypanosomes detected using the conventional parasitological techniques of Giemsa-stained blood smears, wet blood smears and the microhematocrit centrifugation technique (MHCT) was 7% (13/189), 11% (21/189) and 19% (36/189), respectively. However, a multi-species... |
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Palavras-chave: Dromedary camels; Sudan; Trypanosomosis; Trypanosoma evansi; Trypanosoma vivax. |
| Ano: 2017 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4467 |
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| Liyanagunawardena, Nilukshi; Sivakumar, Thillaiampalam; Kothalawala, Hemal; Silva, Seekkuge Susil Priyantha; Battsetseg, Badgar; Dinh Thi Bich Lan; Inoue, Noboru; Igarashi, Ikuo; Yokoyama, Naoaki. |
| Babesia bovis is the most virulent Babesia organism, resulting in a high mortality rate in cattle. The genetic diversity of B. bovis merozoite surface antigens (MSAs), such as MSA-1, MSA-2b, and MSA-2c, might be linked to altered immune profiles in the host animals. The present study aimed to develop type-specific PCR assays for Asian msa-1 genotypes, thereby re-analyzing the genetic diversity of msa-1 in Sri Lanka, Mongolia, and Vietnam. Specific primers were designed for nine Asian msa-1 genotypes, which had been detected based on the phylogeny constructed using msa-1 gene sequences retrieved from the GenBank database. Specificity of the type-specific PCR assays was confirmed using plasmids containing the inserts of msa-1 gene fragments that represent... |
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Palavras-chave: Asia; Babesia bovis; Genetic diversity; Msa-1; Type-specific PCR. |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4489 |
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| Registros recuperados: 36 | |
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